Data from neurons in vivo have shown that spike output can often sustain episodes of high variability. Theoretical studies have indicated that the high conductance state of neurons brought on by synaptic activity can contribute to an increase in the variability of spike output by decreasing the integration timescale of the neuron. In the present work, we were interested in understanding how background synaptic conductance activity alters the interspike interval (ISI) variability of layer III pyramidal cells of the medial entorhinal cortex. We compared ISI variability in pyramidal cells as a result of synaptic current- or conductance-mediated membrane fluctuations. We found that the effects of background synaptic conductance activity on ISI variability depend on the neuron type. In pyramidal cells lacking spike frequency adaptation, the variability increased in relation to a comparable synaptic current stimulus. In contrast, in pyramidal cells displaying spike frequency adaptation, the synaptic conductance stimulus produced lower ISI variability. To understand this result, we constructed a phenomenological model that reproduced the basic properties of these neurons under control and increased leak conductance. We found that leak can change the properties of the neuron by acting as a bifurcation parameter that reduces the afterdepolarization (ADP) and decreases the slope (gain) of the frequency-current relationship, particularly for transient stimuli. A lower gain with the added leak causes a reduction in ISI variability. We conclude that the ability of a high conductance state to increase ISI variability cannot be generalized and can depend on the spike ADP dynamics expressed by the neuron.
RTXI v1.4 Released
This version of RTXI includes new modules, bug fixes, and introduces RTXI’s Patch Clamp Electrophysiology Suite. This marks a new … more →
Bug Fixes for RTXI HDF5 MATLAB Scripts
Due to an issue with the the MATLAB scripts not correctly acquiring data when the data file contained >= 10 … more →
New ways to stay in touch!
RTXI is moving along quickly and we have a few new ways for you to stay in touch with updates … more →
Updated MATLAB functions for HDF5
A tutorial on using HDF5 files and a collection of MATLAB functions are available here: http://www.rtxi.org/docs/RTXIh5.zip RTXI HDF5 Matlab Files … more →
RTXI Update: New COMMENT Datatype for DefaultGUIModel
RTXI v1.2 now has a COMMENT type for Workspaces that also works for DefaultGUIModel. RTXI v1.2 is currently only available … more →
- RTXI v1.4 Released